two-hybrid system

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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bubblebath
Garter
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two-hybrid system

Post by bubblebath » Tue Nov 08, 2005 6:19 am

hi! i want anybody's help re Y2HS. What are experiments which I can perform to confirm intreraction of proteins aside from B-galactosidase assays which confim postive interaction of my protein? I run whole plate assays on my interacting protein but it showed white colonies on x-gal/gal plates. can i use ONPG? is it more sensitive than the x-gal plate assay?thanks

sdekivit
King Cobra
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Post by sdekivit » Tue Nov 08, 2005 5:29 pm

ONPG could be an option

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