iodine stain on potato cell

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xpeix
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iodine stain on potato cell

Post by xpeix » Sun Nov 06, 2005 3:59 pm

Hi,

we recently did a lab where we stained the potato cell with iodine. For some reason, we observed more sacs in the cell after staining...just wondering if this is normal and if the "sacs" we sall might actually be just bubbles(didn't look like bubbles though).

-Thanks in advance

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Post by Poison » Mon Nov 07, 2005 4:01 pm

iodine is the indicator of starch. and starch is a compound found in potato (actually in most of the plant cells).
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Starlight189
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Post by Starlight189 » Mon Nov 07, 2005 6:46 pm

I did that experiment! Well I think it was that experiment...for some reason I couldn't find any sacs at all!
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Post by Navin » Tue Nov 08, 2005 2:38 am

Besides being an indicator of starch, iodine also acts as a stain and helps us to see certain features in more detail. However, ive never seen any "sacs" before. Could you post a photo?
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Post by Navin » Tue Nov 08, 2005 3:26 am

I tried it myself and here are the results:
(you can compare with mine)
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Post by xpeix » Tue Nov 08, 2005 5:03 am

Thanks for replies.

After doing some research, I found that I was looking at amyloplasts. It basically looked like this (but darker, I put too much iodine :( )
Image

The only thing that i don't understand is why i saw more amyloplasts that are smaller after dropping iodine on the slide. (originally in tap water)

please help

-thanx

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Post by Navin » Tue Nov 08, 2005 7:49 am

i guess the iodine stains it, making it more visible. I doubt any reactions took place to make more amyloplasts.
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Post by chetanladdha » Tue Nov 08, 2005 2:04 pm

hey,, water potential = solute potential +pressure potential
is ok,, but from where jelly babies r coming......

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Post by MrMistery » Tue Nov 08, 2005 8:58 pm

I'm guessing that they are harder to stain and you only see them at a higher concentration of iodine. just an opinion
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Starlight189
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Post by Starlight189 » Tue Nov 08, 2005 10:53 pm

chetanladdha wrote:hey,, water potential = solute potential +pressure potential
is ok,, but from where jelly babies r coming......
Bassetts of course :roll:
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Post by MrMistery » Wed Nov 09, 2005 6:51 pm

What does Bassetts mean? Sorry i am from Romania
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Post by Starlight189 » Wed Nov 09, 2005 10:22 pm

Tis ok, It's a sweet company...no one gets my jokes :-(, I think I'll change my signature lol.
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