Use of cDNA when studying splicing

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
nukeman
Garter
Garter
Posts: 19
Joined: Wed May 23, 2012 4:11 pm

Use of cDNA when studying splicing

Post by nukeman » Wed May 15, 2013 11:33 am

Hi

I've read a few studies where the authors aim to study the association of certain proteins to mRNA in a splicing-dependent manner. Mainly they compared samples containing spliced mRNA with cDNA-samples, in a way that to me seemed as if they insinuated that the cDNA samples represented the non-spliced samples. cDNA is, as far as I know, made from mature mRNA which is fully spliced?

Could someone please explain this?

Thanks

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Thu May 16, 2013 9:42 am

can you show some such studies?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

DrGavinSee
Garter
Garter
Posts: 1
Joined: Mon Feb 16, 2015 6:59 am

Re: Use of cDNA when studying splicing

Post by DrGavinSee » Mon Feb 16, 2015 7:06 am

cDNA is reverse transcribe from mature mRNA which only contains exons (coding regions) with introns (non-coding regions) remove. The non-coding region is removed by splicing leaving the exons to be transcribe into proteins. because the proteins are translated from mature mRNA, scientist would associate the protein of interest to the mRNA sequence and therefore reverse transcribing to the cDNA sequence to know the specific gene for that particular protein.

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest