Plasimd prep from frozen cells

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biology_06er
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Plasimd prep from frozen cells

Post by biology_06er » Fri Mar 29, 2013 1:04 am

Hi there,

I read in a protocol that that it is possible to extract plasmid from frozen cells using a kit? How would one do use frozen cells? Would you take a glycerol stock, thaw it out, spin it and use pellet?

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JackBean
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Post by JackBean » Fri Mar 29, 2013 2:07 pm

Just grow the cells, spin them down, freeze and keep at -80°C until used.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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