Over expressing proteins in mammalian cells

Genetics as it applies to evolution, molecular biology, and medical aspects.

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simina
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Over expressing proteins in mammalian cells

Post by simina » Tue Feb 19, 2013 9:24 pm

Can anyone tell me an advantage and a disadvantage of over expressing proteins in mammalian cells to discover their function or localisation?

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JackBean
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Post by JackBean » Wed Feb 20, 2013 1:32 pm

if it's mammalian protein than you have almost native conditions
However, it's not so easy to handle.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

nima12
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Post by nima12 » Mon May 06, 2013 3:08 pm

realy is very hard

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