Gene targeting

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gauthum
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Gene targeting

Post by gauthum » Wed Dec 05, 2012 3:56 pm

hello everyone
I have a question to ask.Can gene targeting be done in human being . I mean a fully grown person.And can anyone say what would be a suitable vector for using.
thanking you
My scientific studies have afforded me great gratification; and I am convinced that it will not be long before the whole world acknowledges the results of my work.-Gregor Mendel

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JackBean
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Post by JackBean » Wed Dec 05, 2012 4:51 pm

what about gene therapy?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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jonmoulton
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Re: Gene targeting

Post by jonmoulton » Thu Dec 06, 2012 11:40 pm

Here's a very recent publication with a few well-known vector examples. Certainly there are other possibilities. This essay also points out some potential pitfalls.

http://www.nature.com/nm/journal/v18/n1 ... -1718.html

I think RNA targeting is an easier way to treat many genetic conditions.

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Post by gauthum » Sun Dec 09, 2012 4:03 pm

hello thank you for your response
Does gene targeting has been successful in human being in much accuracy?
can you please explain in short about cis and trans.Actually i am trying to know more about gene targeting so if you dont mind can you explain.Can retro virus can also be used as vector?
My scientific studies have afforded me great gratification; and I am convinced that it will not be long before the whole world acknowledges the results of my work.-Gregor Mendel

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jonmoulton
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Re: Gene targeting

Post by jonmoulton » Tue Dec 11, 2012 3:54 pm

Very little work has been done in humans because of the strict regulations governing human clinical trials.

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Post by gauthum » Sun Dec 16, 2012 7:24 am

oh ok thank you sir.
My scientific studies have afforded me great gratification; and I am convinced that it will not be long before the whole world acknowledges the results of my work.-Gregor Mendel

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Post by gauthum » Tue Dec 18, 2012 2:03 pm

sir is it possible to remove a specific gene if its locus in the dna is known by gene targeting in humans ?
My scientific studies have afforded me great gratification; and I am convinced that it will not be long before the whole world acknowledges the results of my work.-Gregor Mendel

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jonmoulton
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Re: Gene targeting

Post by jonmoulton » Tue Dec 18, 2012 4:47 pm

It might be possible (in theory, though it would not be allowed) to remove a gene from a gamete or a single-celled zygote. However, in an adult human you would have to remove the gene from every cell, and there is no technology available to alter the DNA in so many cells at once.

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Post by gauthum » Tue Dec 25, 2012 3:16 pm

sir but is it possible to do so in a couple of cell say a hand full of those?
My scientific studies have afforded me great gratification; and I am convinced that it will not be long before the whole world acknowledges the results of my work.-Gregor Mendel

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Re: Gene targeting

Post by jonmoulton » Wed Dec 26, 2012 4:51 pm

Yes. Delivery efficiency to cells in adult tissue will not be 100%. There are likely to be regulatory barriers (legal restrictions). But, in principle it is possible.

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Post by gauthum » Fri Dec 28, 2012 12:47 pm

oh ok sir thank you actually.If you dont mind can you say the ways gene targeting is done.This may be silly question but i am just doing my 12th grade so please tolerate with me sir.
thank you for your time.
My scientific studies have afforded me great gratification; and I am convinced that it will not be long before the whole world acknowledges the results of my work.-Gregor Mendel


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