Need a little help with a purification paper/project

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soisports
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Need a little help with a purification paper/project

Post by soisports » Fri Oct 21, 2005 10:13 pm

Hi,

I need some help involving the purification and characterization of an unknown substance. The substance is from a plant aqueous extract and has inhibitory activity in cell culture (growth and cytokine-induced inflammation). What would be some good preliminary techniques aimed at determing whether the compound is organic/inorganic, a protein/non-protein, a hormone or not... before continuing on with further purification?
Keep in mind that bio-activity has to be assured with each prep. I came up with heating the sample + proteinase K for protein digestion to determine if the compound may be a protein. Perhaps defatting the sample would potentially rule out/in a hormone. What about an easy way of separating organics and inorganics? Phase separation?

Thanks in advance for any help.

Sois

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mith
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Post by mith » Fri Oct 21, 2005 11:45 pm

there are protein based hormones too...
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Enjoying one moment at a time;
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