Can particle size affect spectrophotometry?

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Can particle size affect spectrophotometry?

Post by keetner » Thu Nov 29, 2012 3:24 am

Hey guys,

I'm not sure where else to put this...doesn't seem to fit anywhere else.

But if you have a lipid sample in a cuvette, I know that changing the concentration will affect the absorbance readings. But what about particle size? For example, I was working with two different lipids (phosphatidylcholine and phsophatidylglycerol). We were looking at how these proteins can package together in the presence of apolipoprotein -- specifically, making nanodiscs.

If the lipids are able to pack more tightly together, would that result in a lower absorbance reading? Versus if you have lipids that aren't able to pack more tightly, then wouldn't you have more nanodiscs floating around? I'm not really sure.

Any in put would be greatly appreciated -- thanks!

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Post by JackBean » Thu Nov 29, 2012 10:09 am

Sure, the light is scattered, think about OD600 in microbiology. However, the question is, whether your particles are that big.

Cis or trans? That's what matters.

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