CFU conversion

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Holmes
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CFU conversion

Post by Holmes » Mon Nov 26, 2012 3:45 pm

How do I convert mol/CFU (colony forming unit) to millimol?

I came across a research paper saying:
The absolute amount of ATP per bacterium was calculated by dividing mol ATP ml-1 by c.f.u. ml-1.
There was a drop in ATP level (from 3.39±0.216 to 0.59±0.106 mol c.f.u.-1)

I want the ATP concentration (in bacterium) in millimol.
Will it be just 3.39+-0.216 x 10^3 millimol?
Or do I have to do some conversion for c.f.u?

How to do it?

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JackBean
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Post by JackBean » Mon Nov 26, 2012 8:55 pm

you have to keep it in mmol/CFU
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Holmes
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Post by Holmes » Tue Nov 27, 2012 12:54 am

The problem is, I want to use the concentration value somewhere else and I want it to be specified in millimoles.
Is it okay if I just ignore the cfu and use the value as millimol? Will it remain same? I just want the concentration of ATP inside the bacterial cell.

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JackBean
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Post by JackBean » Tue Nov 27, 2012 10:09 am

Then it's technically per CFU, because each cell theoretically give rise to single colony.
On the other hand, the concentration inside cell will be different from that in media.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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Post by Holmes » Mon Dec 03, 2012 8:22 am

Then I think I should just ignore the cfu and convert Mol to mMol, because it clearly specifies that the concentration is 3.39 mol/cfu.
Thanks a lot.
:)

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