Help with my unknow??

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Joined: Sun Nov 25, 2012 2:58 am

Help with my unknow??

Post by student2012 » Sun Nov 25, 2012 3:15 am

I've been working on my unknown in Microbiology and I'm actually stuck now. I've done gram staining and have determined that my bacteria is gram neg, med rods. However, the tests I've done are coming back negative with two being somewhat quesitonable.

Lactose +/+
Glucose +/+
sucrose +/+
Citrate - green - (so this is -)
Gelatin showed no liquifaction, so this is -
EMB agar - no reaction, so -
Catalase - ( no bubbles)
PAD Agar - not black, so -
Vp -
MR was orange...not sure if - or +
Urease was a peach/rose color
In looking thru Bergy's, I am thinking perhaps E. Coli or Enterobacter Aeorogenes
Any suggestions? I've emailed my professor to see if I should redo it all over again, however he hasnt responded to me email and this report is due by the 28th !!! Any help is much appreciated!
Thanks! :roll:

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Joined: Sun Apr 28, 2013 4:19 am

Post by tweetka1 » Sun Apr 28, 2013 4:58 am

i need help with my unknown
glucose -/-
lactose -/-
sucrose -/-
SIM- all negative
simmon citrate- negative
catalase- positive
MS- negative
blood agar- alpha
urease- negative

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Joined: Sat May 18, 2013 8:19 pm

Post by drfuture » Mon May 20, 2013 3:30 pm

Did you get your sample from tap water?

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