Gram stains

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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yvette
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Gram stains

Post by yvette » Sun Oct 16, 2005 6:49 am

What color is S. aureus if you omit the iodine in gram staining?

I think it would be colorless since the dye would not be trapped inside the cell wall???

Why doesn't methane blue work as a counterstain vice safranin?

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mmiaosmiling
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Post by mmiaosmiling » Fri Oct 28, 2005 6:31 am

if you don't use iodine,the complex substance(CVI dye complex)----which will be held back by the cell wall of Gram positive bacteria----can not form.

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Post by chen.lie » Wed Nov 09, 2005 7:21 pm

exactly so your dye will wash off during the rest of the gram staining process

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