gene order

Genetics as it applies to evolution, molecular biology, and medical aspects.

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repubblica
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gene order

Post by repubblica » Sat Oct 15, 2005 4:11 pm

how can gene order be determined in linkage problems without calculating the recombinant frequency? i dont really understand how to determine gene order by inspection

can anyone help me out?

thanks

biocraz
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Post by biocraz » Sat Oct 15, 2005 7:26 pm

Genetic orders are rather complicated. One way to deal with it is the use of morden technology. i think that will be the way

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zami'87.
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Re: gene order

Post by zami'87. » Wed Oct 19, 2005 5:19 pm

hmm…yeah rather complicated but believe that anyway DNA should be cleaved into fragments by restriction enzymes and then by comparing bands from wild-type and mutant we could determine site of mutation .
The point is to obtain overlapping sites and connect fragments in proper order(use several restriction enzymes). :?
correct me if I’m wrong…. :oops:

Zami’87.
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