Questions on the Sodium Potassium Pump and Enzymes

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JoWillyTso
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Questions on the Sodium Potassium Pump and Enzymes

Post by JoWillyTso » Mon Nov 05, 2012 5:50 pm

Although the Sodium Pump is an ATPase, during it's cycle there is a phosphate group being added and taken away from the protein so surely kinase and phosphotase are involved because an ATPase only hydrolyses ATP?

Also, we are just going over how Enzymes work with their substrates. Am I right in saying all enzymes work in the induced fit way where the enzyme is specific to it's substrate and involves the enzyme and the substrate working at an active site? For example DNA Ligase forms phosphodiester bonds between Okazaki fragments...

What about when one job involves two enzymes ie. DNA Gyrase and DNA Helicase in unwinding the double helix or when an enzyme has two jobs such as DNA Polymerase forming phosphodiester bonds between nucleotides and adding new nucleotides to the RNA Primer.

Also, what is Proeolytic Cleavage?

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JackBean
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Post by JackBean » Tue Nov 06, 2012 11:45 am

proteolytic cleavage is breaking of peptide bond
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

JoWillyTso
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Re: Questions on the Sodium Potassium Pump and Enzymes

Post by JoWillyTso » Tue Nov 06, 2012 2:50 pm

Thanks. :D Any chance of clearing up the other stuff I'm stuck on?

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JackBean
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Post by JackBean » Tue Nov 06, 2012 9:23 pm

The induced fit is basically right. For example DNA Pol has two active sites, try to find out something about proof-reading mechanism.

I don't know about the ATPase, but I don't remember any mention of kinase or phosphatase.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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