Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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I'm transfecting cells right now and I added 100 uL of OptiMEM to a tube with the reporter plasmid and in the other tube I put 1 uL of Lipofectamine. Proceeded with the protocol and plated them on my P19 cells . . . Totally forgot to dilute the Lipofectamine before I mixed the contents after waiting the 5 minutes. Will it still work ??? I know that Lipofectamine forms aggregates but is it really that bad to not dilute the Lipofectamine ?
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