Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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Anita B wrote:Hi,
I have amplified a fragment from plasmid DNA and then purified it.
Now i was to check its quantity- so should i spec it as dsDNA or ssDNA?
Check the way the PCR works... Why for example do you have a 94ºC step?
So you obtain dsDNA, and measure it this way
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