bacterial culture growth

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siddharthsameer
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bacterial culture growth

Post by siddharthsameer » Wed Jul 25, 2012 8:49 am

Hello everyone
I have one question , why do we measure the ph of Preculture.I made a a preculture of e.coli tof 10 for the competent cell, and i was asked to measure the ph of the preculture, and I had 3 precultre in different shake falsk, and the following pH I measured .
shake flask- pH
1 8,2
2 8,4
3 8,6
I dont know the reason behind it, kindly let me know... I am stuck and can not find the answer..I would be highly obliged to you all. waiting for your response.
with regards

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canalon
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Post by canalon » Thu Jul 26, 2012 2:54 am

Never, ever did that. Sounds strange to me. Considering that they will be washed multiple times in plenty of different solutions and buffer any way...
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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