Help with bacteria identification!

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Joined: Sun Jul 08, 2012 5:24 am

Help with bacteria identification!

Post by snowquen08 » Sun Jul 08, 2012 5:29 am

I have a project to finish and i have been trying to research which bacteria it is...

These are my test results

EMB- colony has a purple color, no sheen

TSI- yellow/yellow so it ferments glucose and lactose and/or sucrose

Urease- negative

MIO- M- pos
I - neg
O - neg

I tried to eliminate it down to klebsiella pneumoniae but the only thing wrong is the positive urease...could this just be a delayed reaction or does anyone have any ideas of what this could be?

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Post by Cat » Sun Sep 09, 2012 7:26 pm

It's definitely NOT Klebsiella because it's motile. If I understand your results correctly, your bacteria is:

Lactose/sucrose +ve (EMB, TSA)
H2S -ve (no black in TSA)
Motility +ve
Indole -ve
Ornithine -ve
Urease -ve

Than your choices are among Citrobacter freundii, Hafnia avlei, Serratia rubideae... You either need a list of possibilities or you need to perform more tests.

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