how to make blood lysis buffer

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karenzellie
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how to make blood lysis buffer

Post by karenzellie » Thu May 24, 2012 5:12 pm

Hi,

I was wondering how I should make this recipe:

100mM Tris HCL
100mM EDTA
100mM Nacl
1%SDS

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JackBean
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Post by JackBean » Fri May 25, 2012 5:14 am

What's the problem?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

karenzellie
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Re: how to make blood lysis buffer

Post by karenzellie » Fri May 25, 2012 2:48 pm

I know i need to make stock solutions of the buffers but how do I make this into a solution together to make the appropriate concentrations of each chemical?

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JackBean
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Post by JackBean » Fri May 25, 2012 3:23 pm

You can make 1M Tris, 1M EDTA, 1M NaCl and 10% SDS, mix 100 ml of each and fill up to 1 l.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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