Debate and discussion of any biological questions not pertaining to a particular topic.
Moderators: honeev, Leonid, amiradm, BioTeam
-
michimichi
- Death Adder
- Posts: 68
- Joined: Tue Jan 24, 2012 11:07 pm
Post
by michimichi » Tue Apr 24, 2012 1:33 am
can i confidently detect an e.coli in water just by performing the indole test alone?
-
JorgeLobo
- Coral
- Posts: 424
- Joined: Mon Nov 17, 2008 12:12 am
Post
by JorgeLobo » Tue Apr 24, 2012 2:37 am
No - you'd have to develop a test for indole in mixed culture of direct water inoculation and other bacteria produce indole (from tryptophane) including Vibrio, Pleisomonas, Salmonella, Citrobacter spp.
-
michimichi
- Death Adder
- Posts: 68
- Joined: Tue Jan 24, 2012 11:07 pm
Post
by michimichi » Tue Apr 24, 2012 3:49 am
The indole test was done ofcourse after the presumptive to complete the test, so what i should do is perform further biochemical test other than indole to identify an e.coli. Is API 20E more realible than IMVIC test?
-
JorgeLobo
- Coral
- Posts: 424
- Joined: Mon Nov 17, 2008 12:12 am
Post
by JorgeLobo » Tue Apr 24, 2012 8:38 am
Presumptive? Sorry, I thought you were suggesting unique protocol to identify directly presence of E. coli in water. What is the purpose then? If a one-time experiment, use API.
-
michimichi
- Death Adder
- Posts: 68
- Joined: Tue Jan 24, 2012 11:07 pm
Post
by michimichi » Tue Apr 24, 2012 2:58 pm
ah ok, its not practical to use API always,,, its more expensive than the IMVIC but API can give rapid results..thnks sir
-
JorgeLobo
- Coral
- Posts: 424
- Joined: Mon Nov 17, 2008 12:12 am
Post
by JorgeLobo » Tue Apr 24, 2012 3:07 pm
You could do it the old way - smell, colony morphology on EMB and Mac
Who is online
Users browsing this forum: No registered users and 1 guest
