3' end of a PCR primer

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Sue4
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3' end of a PCR primer

Post by Sue4 » Mon Apr 23, 2012 7:17 pm

Why it is necessary to avoid more than three G or C nucleotides at the 3' end of a PCR primer?

wbla3335
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Re: 3' end of a PCR primer

Post by wbla3335 » Mon Apr 23, 2012 11:29 pm

Too many Gs or Cs can encourage mispriming. The ones that match can bind strongly, thereby increasing the chances that mismatches at other positions are tolerated.

charless
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Post by charless » Tue Apr 24, 2012 3:54 pm

It's good, also see similar at deleted link
Last edited by JackBean on Mon May 14, 2012 11:14 am, edited 1 time in total.
Reason: unrelated link removed

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