Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
Moderators: honeev, Leonid, amiradm, BioTeam
- Posts: 16
- Joined: Tue Apr 10, 2012 9:55 am
Why it is necessary to avoid more than three G or C nucleotides at the 3' end of a PCR primer?
- Posts: 227
- Joined: Thu Aug 17, 2006 7:20 am
Too many Gs or Cs can encourage mispriming. The ones that match can bind strongly, thereby increasing the chances that mismatches at other positions are tolerated.
- Posts: 2
- Joined: Tue Apr 24, 2012 3:35 pm
It's good, also see similar at deleted link
Last edited by JackBean
on Mon May 14, 2012 11:14 am, edited 1 time in total.
Reason: unrelated link removed
Who is online
Users browsing this forum: No registered users and 4 guests