Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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Why it is necessary to avoid more than three G or C nucleotides at the 3' end of a PCR primer?
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Too many Gs or Cs can encourage mispriming. The ones that match can bind strongly, thereby increasing the chances that mismatches at other positions are tolerated.
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It's good, also see similar at deleted link
Last edited by JackBean
on Mon May 14, 2012 11:14 am, edited 1 time in total.
Reason: unrelated link removed
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