Hard Question

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mxnick262
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Hard Question

Post by mxnick262 » Wed Apr 04, 2012 7:57 pm

You have performed yeast two hybrid assay to identify proteins that interact with superman and identified a gene coding for kryptonite that interacts with superman. What experiments you will perform to demonstrate that the superman interacts with kryptonite in-vivo (inside nucleus in the cell)?

Give atleast two examples.

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JackBean
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Post by JackBean » Thu Apr 05, 2012 2:08 pm

Where is your attempt to solve this "hard" problem? Have you not done any such methods in your course?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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Post by mxnick262 » Tue Apr 17, 2012 1:28 am

Please post something mildly useful. Methods that I have considered: protein-complex immunoprecipitation coupled with the use of some sort of molecular beacon? Guidance please.

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JackBean
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Post by JackBean » Tue Apr 17, 2012 6:53 am

I thought you were asked for in vivo method, not in vitro
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

mxnick262
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Post by mxnick262 » Tue Apr 17, 2012 3:54 pm

Yea you are right. This question has me stumped.

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Post by mxnick262 » Tue Apr 17, 2012 11:23 pm

BiFC. It's really a hypothetical question so even though this method has absolutely nothing to do with what we have learned this semester, it may still be right.

Still need one more method. GFP is to similar...

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Post by mxnick262 » Tue Apr 17, 2012 11:33 pm

FRET*

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JackBean
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Post by JackBean » Wed Apr 18, 2012 6:25 am

Here you go, you have answered your question by your own.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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