Designing Primer for over lapping PCR
Moderators: honeev, Leonid, amiradm, BioTeam
-
Rezaulbiox
- Garter
- Posts: 1
- Joined: Tue Feb 28, 2012 7:20 am
Designing Primer for over lapping PCR
Hello everyone,
I am trying to amplify a little bit long DNA of 6.72kb. I designed primer within 100 bp upstream and downstream of this DNA but failed to get the DNA by PCR. Some one suggested me to amplify the DNA by overlapping PCR. I do not know how to design the primer for overlapping PCR. I usually used Primer Premier 6 as primer designing software.
You are highly appreciated to give me some guidelines to design overlapping primer by using Primer Premier 6.
Thanking you
Khan
I am trying to amplify a little bit long DNA of 6.72kb. I designed primer within 100 bp upstream and downstream of this DNA but failed to get the DNA by PCR. Some one suggested me to amplify the DNA by overlapping PCR. I do not know how to design the primer for overlapping PCR. I usually used Primer Premier 6 as primer designing software.
You are highly appreciated to give me some guidelines to design overlapping primer by using Primer Premier 6.
Thanking you
Khan
Who is online
Users browsing this forum: No registered users and 11 guests