Please identify this structure. THX.

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fangpaul74
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Please identify this structure. THX.

Post by fangpaul74 » Thu Feb 23, 2012 6:15 pm

Hi everyone,
I tested one new purified antibody. and Immunostained U2OS cells after fixing by methanol. Please give me some suggestions about this structure. Thanks.
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Vesicles.jpg

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JackBean
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Post by JackBean » Thu Feb 23, 2012 6:22 pm

Seems like cytoskeleton to me, but you will need colocalization to confirm.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

fangpaul74
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Re: Please identify this structure. THX.

Post by fangpaul74 » Fri Feb 24, 2012 3:50 pm

BTW, It doesn't co-localize with F-actin and microtubes. It seems look like some kind of vesicles. But these vesicles don't like endosomes, golgi or ER, too.

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JackBean
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Post by JackBean » Fri Feb 24, 2012 7:35 pm

maybe you could say that in your first post :roll: Anything else you have to add?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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