kovacs reagent

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michimichi
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kovacs reagent

Post by michimichi » Tue Feb 07, 2012 3:42 am

1. what is the storage temp.for prepared Kovacs reagent
2. can i add the Kovacs reagent directly to positive Lauryl Tryptose Broth to detect e.coli or do i have to use Tryptophan broth?

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canalon
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Post by canalon » Tue Feb 07, 2012 3:44 am

1. We used to put it at 4°C, and never had problems
2. You have to use tryptophan broth to avoid false negative.
Patrick

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any proof. (Ashley Montague)

michimichi
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Re: kovacs reagent

Post by michimichi » Tue Feb 07, 2012 3:57 am

can an indole test (kovacs) alone can prove that a water sample has e.coli ?
about the storage of kovacs - i stored it at room temp , so it isnt acceptable.?
can i use peptone water as a subtitute for tryptophan? :-)

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canalon
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Post by canalon » Tue Feb 07, 2012 1:41 pm

No.
Don't know, but considering the ingredients, I imagine that the problem is more the stability of the coloring agent. It might be OK.
That is what I used. Peptone broth has enough aa including tryptophane that it was considered as a good substrate for indole reaction.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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