Why RT-PCR

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charisma
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Why RT-PCR

Post by charisma » Sun Jan 29, 2012 4:26 pm

Hi,
I've done gene transformation to rice.To confirm that new gene has been transferred to rice I've already done genomic DNA PCR and the result showed the existence of the new gene but My supervisor asked me to do RT-PCR (Reverse transcription) too. Could someone please let me know what are RT-PCR preferences to genomic DNA? When I got the desire result by total genomic PCR, what's the point to do RT-PCR?
Thanks.

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canalon
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Post by canalon » Sun Jan 29, 2012 4:57 pm

To see whether your gene is expressed, I would guess.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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JackBean
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Post by JackBean » Mon Jan 30, 2012 9:46 am

yep, your gene may be silenced after incorporation or your promoter may not work properly, thus you need to check expression of your transgene.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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