gene-break transposons
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jonmoulton
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gene-break transposons
This is gene engineering at the edge: "implementing gene-break transposons to create expression-tagged, revertible mutations in zebrafish genes". http://zfishbook.org/
Here's a description of the technology:
Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC. In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8.
http://www.nature.com/nmeth/journal/v8/n6/full/nmeth.1606.html
Clark KJ, Argue DP, Petzold AM, Ekker SC. zfishbook: connecting you to a world of zebrafish revertible mutants. Nucleic Acids Res. 2011;1–5. doi:10.1093/nar/gkr957
http://nar.oxfordjournals.org/content/early/2011/11/07/nar.gkr957.full
Sometimes you just gotta post it 'cause it's cool.
Here's a description of the technology:
Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC. In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8.
http://www.nature.com/nmeth/journal/v8/n6/full/nmeth.1606.html
Clark KJ, Argue DP, Petzold AM, Ekker SC. zfishbook: connecting you to a world of zebrafish revertible mutants. Nucleic Acids Res. 2011;1–5. doi:10.1093/nar/gkr957
http://nar.oxfordjournals.org/content/early/2011/11/07/nar.gkr957.full
Sometimes you just gotta post it 'cause it's cool.
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