gene-break transposons

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gene-break transposons

Post by jonmoulton » Wed Jan 11, 2012 11:02 pm

This is gene engineering at the edge: "implementing gene-break transposons to create expression-tagged, revertible mutations in zebrafish genes".

Here's a description of the technology:
Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC. In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8.

Clark KJ, Argue DP, Petzold AM, Ekker SC. zfishbook: connecting you to a world of zebrafish revertible mutants. Nucleic Acids Res. 2011;1–5. doi:10.1093/nar/gkr957

Sometimes you just gotta post it 'cause it's cool.

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