N/C terminus

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biology_06er
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N/C terminus

Post by biology_06er » Sun Dec 04, 2011 5:56 am

Hi there,

I recently cloned my gene of interest into a Pet32a vector containing a GFP protein..sites BgII/EcoR1. I then fused my gene containing EcoR1/Xho1 sites. When I look at the Pet vector map, am I right in saying my gene is cloned downstream of GFP and attached to the C TERMINUS of GFP.

cheers,
b_06er

N.B...this is the map I am looking at (I can't seem to attach the file hence the web link)..pet32a-c
http://www.merck-chemicals.co.uk/life-s ... ed+Kingdom

biology_06er
Coral
Coral
Posts: 260
Joined: Tue Mar 21, 2006 9:33 am

Post by biology_06er » Sun Dec 04, 2011 6:05 am

oh and would my thioredoxin tag be on the N terminal of GFP/any gene cloned into the MCS

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