ELISA graph plot

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biology_06er
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ELISA graph plot

Post by biology_06er » Sat Nov 26, 2011 8:00 am

Hi there,

I recently carried out my first ELISA, and to each well I added varying concentrations of my antibody to my protein. I started with a 1:200 dilution and then titrated 2 fold so ended up having 1:200, 1:400, 1:800, 1:1600, 1:3200, 1:6400, 1:12800, 1:25600, 1:51200, 1:102400, 1:204800, 1:409600 and then added each conc. to a well, and read plate.

I got readings and now have to plot them on a graph..just wondering what do I write on the X-axis...Y would be absorbance and X would would be concentration of antibody but how do I write concentration in terms of an X-axis...hope that makes sense!?

Thanks
biology_06er

biology_06er
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Post by biology_06er » Sat Nov 26, 2011 8:31 am

Could I just plot it as 200, 400, 800 etc and write as a 1/serum dilution (x100) (I know there a various ways as google shows but just wondering is there a standard one that works/looks best)

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JackBean
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Post by JackBean » Sat Nov 26, 2011 10:46 am

the best is probably the logarithmic scale as 2, 4, 8 etc. and description as 1/100x dilution or just calculate it as 0.05, 0.025 etc.
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