Using the pipette

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Using the pipette

Post by SWEngineer » Sat Nov 19, 2011 3:38 pm

I just want to confirm the usage of a 50-200 ul autopipette. If I want to obtain a volume of 0.1 ml using such pipette, will the setting used be 050?


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Post by ladilov » Sun Nov 20, 2011 11:38 am

Setting should be in 100ul.

100ul = 0.1ml

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Post by daniel.kurz » Mon Nov 21, 2011 4:57 am

For those novices out there, the pipette companies have made things easy by maintaining a system of showing exactly the amount in uL that you would like to pipette. This is opposed to using a percentage of an amount in mL. So in a p2 pipette set to 2 that equals 2 uL and a p1000 set to 375 will deliver 375 uL.

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Post by JackBean » Mon Nov 21, 2011 7:30 am

it has ever been in percentage?

Cis or trans? That's what matters.

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Post by nestaB » Tue May 22, 2012 11:25 pm

Since your pipette is 50-200ul you should be able to adjust the setting to 100ul volumes. Assuming everything is calibrated you'll get accurate results. Also it helps to use tips that have graduation marks...usually a line on the tip that marks 10ul, 50ul, 100ul...etc.

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