ion exchange chromatography

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biology_06er
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ion exchange chromatography

Post by biology_06er » Wed Nov 02, 2011 3:20 am

Hi there,

I plan on doing IEC tomorrow and need to dialyze my protein to keep a negative charge...the plan is to dialyze in Tris pH 8 (pi of my protein is 6) does that sound right...I don't have to add NaCl to the buffer right (NaCl is what I'm eluting with)..I seem to have written in my book "add NaCl to Tris and dialzye O/N" but I'm pretty sure it's wrong..

aptitude
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Post by aptitude » Wed Nov 02, 2011 6:20 am

Yes, eluting buffer would come afterwards.

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