About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.
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- Posts: 34
- Joined: Sat May 08, 2010 1:24 pm
If we forget to do the iodine step for example when doing gram staining, provided that the microorganism we have is gram positive, what will the colour of the stained microorganism in this case be? Will it be with NO colour or will it be pink-red (the colour of the gram negative)? Why is that?
- Inland Taipan
- Posts: 3909
- Joined: Thu Feb 03, 2005 2:46 pm
- Location: Canada
What is the role of iodine? (look it up)
What does the secondary coloration do? How discriminant is it?
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
- King Cobra
- Posts: 635
- Joined: Thu Feb 14, 2008 7:40 pm
"Will it be with NO colour or will it be pink-red (the colour of the gram negative)? " - pink-red
"Why is that?" - look up the function of iodine in gram staining. What happens when it's added to crystal violet?
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