Is this the way we sterilize a wire loop?

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SWEngineer
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Is this the way we sterilize a wire loop?

Post by SWEngineer » Sat Oct 22, 2011 1:01 pm

Do we sterilize a wire loop by putting it in a flame until it becomes red?

Thanks.

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JackBean
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Post by JackBean » Sat Oct 22, 2011 2:39 pm

are you cheking our knowledge?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

daniel.kurz
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Post by daniel.kurz » Mon Oct 24, 2011 3:30 am

Guessing from the number of posts by that user that are all very basic, the person is not in microbiology. No you do not need to heat it till it becomes red. A short duration ~1 second to be absolutely certain will suffice. The microbes are killed rapidly by the quick change from cold to hot to relative cold. The shock kills them. Kinda like taking a human to volcanic lava and then to the Greenland ice shelf.

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canalon
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Post by canalon » Wed Oct 26, 2011 1:53 am

Good practice in my microbio lab is to leave it until red. Particularly useful if you have clumps that are attached, a simple heating will not kill all bacteria. And I 'd rather do a bit of overkill, than having to redo an experiment... Just saying.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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JackBean
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Post by JackBean » Wed Oct 26, 2011 3:26 pm

agree with canalon
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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