## Cell treatment, various concentrations from a stock solution

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### Cell treatment, various concentrations from a stock solution

Hi everyone,

I am working with smooth muscle cells and resveratrol, a molecule found in grape skins which has been proven to reduce hypertension and to have various antioxidant effects.

I need to treat each cell culture with different concentrations of this molecule (diluted in DMSO).

I am planning on making my stock solution of 1mM in 1mL.

The MW of resveratrol being 228, I would then need 2.28mg of it and dilute it into 1mL of DMSO.

The next step is the one I am not sure of.

The 3 concentrations I will be using are 10uM, 50uM and 100uM. So small concentrations.

My concern is: what is the calculations I need to perform to know how much volume of the stock solution (1mM) in order to get those concentrations?

If someone can enlighten me that would be great,

Thank you,

I am working with smooth muscle cells and resveratrol, a molecule found in grape skins which has been proven to reduce hypertension and to have various antioxidant effects.

I need to treat each cell culture with different concentrations of this molecule (diluted in DMSO).

I am planning on making my stock solution of 1mM in 1mL.

The MW of resveratrol being 228, I would then need 2.28mg of it and dilute it into 1mL of DMSO.

The next step is the one I am not sure of.

The 3 concentrations I will be using are 10uM, 50uM and 100uM. So small concentrations.

My concern is: what is the calculations I need to perform to know how much volume of the stock solution (1mM) in order to get those concentrations?

If someone can enlighten me that would be great,

Thank you,

### Re: Cell treatment, various concentrations from a stock solution

tsao wrote:I am planning on making my stock solution of 1mM in 1mL.

Do you mean 1 mM or 1 mmol in 1 ml?

Either use simple formula c1V1 = c2V2

or calculate, how many times will you dilute the solution (1 mM/100 uM = 10-times) and so many times lower volume you will add (e.g. 1 ml to 10 ml of solution).

http://www.biolib.cz/en/main/

*Cis*or*trans*? That's what matters.1mM stock means 1mmole/L or 1umole/ml, so if you want to use this concentration for the stock solution, you will need the total of 160ml to make working solutions. You will need a more concentrated stock. I would suggest 1M solution (1mole/L or 1mmole/ml or 1umole/ul) make 1ml of it. You would need 10ul, 50ul, and 100ul diluted to 1ml each solution respectively.

You also made a mistake in initial calculation.

"The MW of resveratrol being 228, I would then need 2.28mg of it and dilute it into 1mL of DMSO."

If MW = 228 g/mole (or 228mg/mmole or 228ug/umole...) and you need 1mM stock, you will need 1umole/ml. Thus, 228ug or 0.228mg (not 2.28mg).

I have another question concerning concentrations/volumes.

If I have a 10mM stock solution of X lets say, into 1mL.

If I take 5uL out of it and put it into 5mL of another solution.

The new concentration of X into the 5mL solution will be 10uM.

This is correct I believe, it was given to me by a professor, but I don't get the math behind it.

Can you guys maybe help me on that?

Thanks

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