## Western Blot Protein Length

**Moderators:** Leonid, amiradm, BioTeam

### Western Blot Protein Length

Hello Forum,

please take a look at this image

http://imageshack.us/photo/my-images/98/0bigg.jpg/

It shows a Western blot of an extract of proteins.

My question is how can you determine the length of both proteins A and B graphically?

Could you please help me?

please take a look at this image

http://imageshack.us/photo/my-images/98/0bigg.jpg/

It shows a Western blot of an extract of proteins.

My question is how can you determine the length of both proteins A and B graphically?

Could you please help me?

the mobility (expressed as distance of band from the start) is proportional to log M_r

So, if you want to have it precise, measure distances from start to respective bands, calculate log of their molecular weight and put into graph. By calculating the equation, you can calculate M_r of your proteins

So, if you want to have it precise, measure distances from start to respective bands, calculate log of their molecular weight and put into graph. By calculating the equation, you can calculate M_r of your proteins

http://www.biolib.cz/en/main/

*Cis*or*trans*? That's what matters.### Re: Western Blot Protein Length

Thank you for your answer.

I have some questions left.

What do you mean by M_r ?

How can I put them into the graph? What is the equation?

I have some questions left.

What do you mean by M_r ?

How can I put them into the graph? What is the equation?

### Re: Western Blot Protein Length

Thank you for your answer.

I need help with Excel.

Can you tell me what I have to type in?

Do you use any kind of messengers so I can ask you in a faster way?

I need help with Excel.

Can you tell me what I have to type in?

Do you use any kind of messengers so I can ask you in a faster way?

### Re: Western Blot Protein Length

Why do I have to use the log of MW ?

### Re: Western Blot Protein Length

Thank you.

I read on other websites that there is another way to solve this.

They basically follow the same procedure but instead of only measuring the distance from the starting point they divide the length of the protein by the distance from the starting point.

So you have another coordinate system with the log(MW) on the x-axis and the division of distance over length on the y-axis.

Which way do you recommend? Yours or the above mentioned?

I read on other websites that there is another way to solve this.

They basically follow the same procedure but instead of only measuring the distance from the starting point they divide the length of the protein by the distance from the starting point.

So you have another coordinate system with the log(MW) on the x-axis and the division of distance over length on the y-axis.

Which way do you recommend? Yours or the above mentioned?

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