Genetics as it applies to evolution, molecular biology, and medical aspects.
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- Joined: Thu Aug 25, 2011 6:21 pm
- Location: Federal University of Viçosa, Viçosa-MG, Brazil.
Mutations in APC gene can produce an excess of beta-catenin. We know that the beta-catenin can interage with the gene HIF1 in these conditions. My question is if in normal cells when the beta-catenin is produced there are interaction with this gene too. Sorry by my english.
Hermanfialho wrote:Mutations in APC gene can produce an excess of beta-catenin. We know that the beta-catenin can interage
you mean interact, dude.
Hermanfialho wrote:with the gene HIF1
you mean HIF1 protein, not gene
Hermanfialho wrote:in these conditions. My question is if in normal cells when the beta-catenin is produced there are interaction with this gene too.
well, whats a 'normal' cell...because not all normal cells produce this protein, so you want to know this too. As I understand it, to calculate the Kd (dissociation constants), you need to know the concentration of both proteins in your normal cell.
Hermanfialho wrote: Sorry by my english.
thats fine, keep at it!
Finally, my guess is that they will, but where do you get your information from that they bind at all? Anyway, to cut to the chase, One type of experiment you might like to try is:
In-vivo crosslinking of protein complexes using photo-reactive amino acid analogs as introduced in 2005 by researchers from the Max Planck Institute. In this method, cells are grown with photoreactive diazirine analogs to leucine and methionine, which are incorporated into proteins. Upon exposure to ultraviolet light, the diazirines are activated and bind to interacting proteins that are within a few angstroms of the photo-reactive amino acid analog.
http://en.wikipedia.org/wiki/Methods_to ... teractions
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