plasmid cut and uncut

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biology_06er
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plasmid cut and uncut

Post by biology_06er » Thu Aug 18, 2011 2:48 am

Hi there,

I have to bands on my gel..one uncut plasmid and one cut...they were more or less the same size with the cut being slightlyyy bigger..(looking at them both compared to the ladder)....Just wondering if there is a reason for this?

protold
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Re: plasmid cut and uncut

Post by protold » Thu Aug 18, 2011 5:48 am

Yes, plasmids show different mobility depending of their conformation. the fastest= supercoiled. above this linear digested and above these relaxed nicked circular form check any manual to confirm. Buena suerte

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JackBean
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Post by JackBean » Mon Oct 31, 2011 4:03 pm

the uncut plasmid should have more than one bands. I have seen usually two, but I have read about up to 5. Onthe other hand the cut vector should show sharp single band.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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