has anyone tried the acrosome reaction in vitro?

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deedeesofia
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has anyone tried the acrosome reaction in vitro?

Post by deedeesofia » Wed Aug 31, 2005 8:02 pm

I am trying to induce the axrosome reaction in vitro, and to check whether they are reacted or not, I stain with FITC-PNA, which should only stain intact acrosomes.
I have looked through all the papers, the entire internet, and asked a couple of dusty old professors, but noone has given me a decent protocol, as I seem to either kill the sperm beofre inducing the reaction, or they just dont react, as thye all stain for the acrosome. the situation is drastic, i need the experiment to be done for my thesis, which I am writing these days. has anyone done this in mice and it has worked??pleeeease tell me what to do!!

yours desperate,
deedee

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