How to get one species whole (unknown) gene sequences?

Genetics as it applies to evolution, molecular biology, and medical aspects.

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zhouxc
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How to get one species whole (unknown) gene sequences?

Post by zhouxc » Thu Jul 21, 2011 6:21 am

How to get one species whole (unknown) gene sequence based on other species known gene?

Thanks!

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JackBean
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Post by JackBean » Mon Aug 01, 2011 11:17 am

by BLASTing the gene sequence against the other species' genome?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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JackBean
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Post by JackBean » Mon Aug 01, 2011 11:17 am

or if you mean not in silico, then something like library hybridization or degenerated primer use
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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