Growing MDCK Cells on Coverslips

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mutant11
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Growing MDCK Cells on Coverslips

Post by mutant11 » Mon Jul 04, 2011 11:55 pm

Hi all,

Does anyone work with MDCK cells? I want to seed them onto glass coverslips and have been using glass coverslips coated with poly-L-lysine. The cells sometimes do not stick well to the coverslips and I have found that the transient transfection rate is lower on the coated glass slides than just doing the transfections in the plastic cell culture wells.

How do you usually seed MDCK cells onto coverslips? Do you just use the coverslips alone? Do you add collagen? (how much etc)

Thanks!

Jessica888
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Re: Growing MDCK Cells on Coverslips

Post by Jessica888 » Tue Aug 09, 2011 12:45 am

It is most likely the coating of your coverslips is not good. The lower transfection rate is due to the lower survival rate of your transfected cells on poor coating. I know someone uses coated coverslips from neuvitro.com and got good culture results.

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