How does chloroform bind to proteins and lipids?

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Melstmu2010
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How does chloroform bind to proteins and lipids?

Post by Melstmu2010 » Wed Jun 22, 2011 4:01 pm

I am aware that in PCR Chloroform from either Phenol: Chloroform: Isoamyl or Chloroform: Isoamyl binds to protein and lipids causing them to precipitate. Where exactly does the Chloroform bond (mechanism, please)??

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JackBean
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Post by JackBean » Thu Jun 23, 2011 8:34 am

the chloroform is hydrophobic and thus it sticks to the hydrophobic amino acid side chains and make thus proteins to denature
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Melstmu2010
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Re: How does chloroform bind to proteins and lipids?

Post by Melstmu2010 » Thu Jun 23, 2011 8:48 pm

Thank you!!

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