WB - Transferring REALLY big proteins

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aalvesdu
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WB - Transferring REALLY big proteins

Post by aalvesdu » Mon Jun 13, 2011 1:26 pm

Hi,

I've been trying to detect ATR and ATM by western blot without much success. Aside the possible issues with the antibodies, I'm unsure of what the best transfer conditions are for proteins of this size (300 and 350kDa, respectively). Right now I'm doing it for ~75min but that might be still insufficient for these. Anyone has any experience with this?

Thanks!

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