Fish immunogenomics

Genetics as it applies to evolution, molecular biology, and medical aspects.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Jevandrix
Garter
Garter
Posts: 2
Joined: Thu Jun 02, 2011 3:05 am

Fish immunogenomics

Post by Jevandrix » Thu Jun 02, 2011 3:11 am

Hi everyone,

I am doing a transcriptomic study on fish immunogenomics to study expressed immune genes, which involves fish-pathogen challenge. To do this I would like to know the parameters that I need consider. Especially, what is the appropriate concentration of bacteria to be used? Is strain-specific or fish species specific or both.

For additional info I am will be working on Asian seabass Lates Calcarifer challenged with live V. alginolyticus. But I do not know what concentration to use, is it dependant on LD-50, Lethal concentration or something else?

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Thu Jun 02, 2011 3:52 am

It usually depends on the bacteria, the fish and the mode of infection. So basically I think you will have to bite the bullet and prepare a test to define the paramater of your experimental model. That said, there is no need to kill the fish if that is not the general expected outcome of your experiment. A simple stable infection might ne enough. It depends on what you want.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest