Organism-pathogen challenge protocol

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Jevandrix
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Organism-pathogen challenge protocol

Post by Jevandrix » Thu Jun 02, 2011 3:10 am

Hi everyone,

I am doing a transcriptomic study on fish immunogenomics to study expressed immune genes, which involves fish-pathogen challenge. To do this I would like to know the parameters that I need consider. Especially, what is the appropriate concentration of bacteria to be used? Is strain-specific or fish species specific or both.

For additional info I am will be working on Asian seabass Lates Calcarifer challenged with live V. alginolyticus. But I do not know what concentration to use, is it dependant on LD-50, Lethal concentration or something else?

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