Why do a 96-well plate?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 5
Joined: Tue Jun 14, 2005 12:57 pm

Why do a 96-well plate?

Post by bht » Sat Aug 27, 2005 7:48 am

Creating a B-cell hybridoma
After fusing two types of cells, myeloma cell and b-cells, why do we need to plate them onto the 96-well plate instead of just plating them onto a normal flask? Note that the next protocol was to screen for the specificity of the antibodies obtained using ELISA

User avatar
Posts: 193
Joined: Wed Jun 08, 2005 3:31 pm
Location: Somewhere between those molecules

Post by chemistry_freako » Mon Sep 05, 2005 12:29 pm

I thought it's so we can get one cell per well, so we can get MONOclonal antibodies - from like the one cell in the well - the produce one clone, as compared to the fact that if grown in a flask, this would not be the case


Post Reply

Who is online

Users browsing this forum: No registered users and 6 guests