Why do a 96-well plate?

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bht
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Why do a 96-well plate?

Post by bht » Sat Aug 27, 2005 7:48 am

Creating a B-cell hybridoma
After fusing two types of cells, myeloma cell and b-cells, why do we need to plate them onto the 96-well plate instead of just plating them onto a normal flask? Note that the next protocol was to screen for the specificity of the antibodies obtained using ELISA

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chemistry_freako
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Post by chemistry_freako » Mon Sep 05, 2005 12:29 pm

I thought it's so we can get one cell per well, so we can get MONOclonal antibodies - from like the one cell in the well - the produce one clone, as compared to the fact that if grown in a flask, this would not be the case
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