How to change the genetic information of a virus?

Genetics as it applies to evolution, molecular biology, and medical aspects.

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ZEGSUS
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How to change the genetic information of a virus?

Post by ZEGSUS » Thu Apr 14, 2011 1:45 pm

How to change the genetic information of a virus?

Babybel56
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Post by Babybel56 » Sun Apr 24, 2011 8:39 am

You mean mutate it's genome? Same way you'd do it with anything else: using mutagens like radiation or chemicals I'd imagine.

ZEGSUS
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Post by ZEGSUS » Tue Apr 26, 2011 11:49 am

What kind of mutagen?

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Post by Babybel56 » Tue Apr 26, 2011 11:58 am

Buddy your guess is as good as mine. X-rays or UV light would do it, or sodium azide, benzene and any number of other chemicals.

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Post by ZEGSUS » Thu Apr 28, 2011 7:03 pm

And how to work with viruses?Where I can found them?How to make them to grow and so on and so on.Is there a some guide?

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canalon
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Post by canalon » Thu Apr 28, 2011 9:35 pm

A good formation in virology in your friendly neighbourhood university?
The variety of viruses is huge they infect eukaryotes, prokaryotes and even other viruses. To study any virus you nedd to be able to grow the host (or some cells of that host, and not all of them can be infected by any virus of said host) then to have method to extarct and purify the virus (high speed centrifuge and chemicals of various density). It is not generally something that you can read a book about and start doing in your garage 5 minutes later. And then comes the elves of safety (which have gone slightly mad a long time ago :))
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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Post by ZEGSUS » Fri Apr 29, 2011 3:57 am

Ok ok.I want to work with flu viruses because I think that I will find them easy.Do you have some guide for them?

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canalon
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Post by canalon » Mon May 02, 2011 2:02 pm

[sarcasm]Great choice[/sarcasm]. It is actually quite a complicated family of virus (There are 3 main types: A, B and C, and there are many subtypes among those).

I really suggest that if you are truly interested you take good classes in the subject. You could start by reading a textbook on the subject, I cannot recommend one, as it is not my specialty, but you can even look for free articles in pubmed and books in the book section of the same database: http://www.ncbi.nlm.nih.gov/books/
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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Post by ZEGSUS » Tue May 03, 2011 6:56 pm

Thanks.I was thinking that with fly will be easy because I will find it easy.What lab equipment will be needed for working with viruses?

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JackBean
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Post by JackBean » Thu May 12, 2011 7:28 am

something like this post-131521.html#p131521 ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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