how to storage mitochondrial at 37℃ for 1 hour,and maintain

Discussion of all aspects of cellular structure, physiology and communication.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
yissa
Garter
Garter
Posts: 14
Joined: Wed Mar 17, 2010 2:09 pm

how to storage mitochondrial at 37℃ for 1 hour,and maintain

Post by yissa » Wed Apr 13, 2011 3:54 pm

how to store mitochodrial for 1 hour at 37℃
I have isolated the mitochondrial from rat liver by sigma kit,then dye them with TMRM.I find the mitochodrials are all at a high level of activity.But when the mitochondrial is transfered to room tempreture for 1 hour,they can't maintain their activity.

how to storage mitochondrial at 37℃ for 1 hour,and maintain their normal state.

Im sure someone here is more expert than I am

thanks in advance

yissa
Garter
Garter
Posts: 14
Joined: Wed Mar 17, 2010 2:09 pm

Re: how to storage mitochondrial at 37℃ for 1 hour,and maintain

Post by yissa » Thu Apr 14, 2011 3:46 am

help

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Tue Sep 20, 2011 1:57 pm

what about providing enough substrates? (and removing products, but that would be little difficult)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Post Reply

Who is online

Users browsing this forum: amandataylor, Baidu [Spider] and 13 guests