Help in expressed sequence tag

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angellee0113
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Help in expressed sequence tag

Post by angellee0113 » Tue Mar 29, 2011 3:53 am

Hello, can anyone explain to me whats the purpose for EST ( Expressed sequence tag ) and the methods please??? I couldn't understand after i read my notes... thanks a bunch !!! =))

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canalon
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Post by canalon » Tue Mar 29, 2011 3:59 am

Basically fragment obtained by Reverse transcriptase PCR on expressed mRNA are sequenced with your method of choice. Since they can be quite long (sanger method gives 500 to 800 nt), their sequence is unique and can then be detected in the genome. This allow the placement of expressed genes in the different chromosomes.

I you want more details, you can start from the eil wikipedia and look at the reference therin or come back with more detailed questions

Good luck
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

angellee0113
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Post by angellee0113 » Tue Mar 29, 2011 4:30 am

Thanks for your information =)

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