how to solve problems on gels?

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moonj88
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how to solve problems on gels?

Post by moonj88 » Mon Mar 21, 2011 6:18 pm

Hi,

How can I solve,

1)When two molecules are comigrating, so they are shown as one band,
2)When a molecule is much smaller than the others so that it runs off of a gel

And one more comment, is there any way to adjust those problems without
affecting other normally shown bands?

Thanks a lot

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canalon
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Post by canalon » Mon Mar 21, 2011 10:22 pm

1) change migration conditions so you are not separating by size only (charge, pH,...)
2)longer gel or shorter migration of different concentration of gellling.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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JackBean
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Post by JackBean » Mon Mar 21, 2011 11:05 pm

you could ask on your previous post...
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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