how to solve problems on gels?

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how to solve problems on gels?

Post by moonj88 » Mon Mar 21, 2011 6:18 pm


How can I solve,

1)When two molecules are comigrating, so they are shown as one band,
2)When a molecule is much smaller than the others so that it runs off of a gel

And one more comment, is there any way to adjust those problems without
affecting other normally shown bands?

Thanks a lot

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Inland Taipan
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Post by canalon » Mon Mar 21, 2011 10:22 pm

1) change migration conditions so you are not separating by size only (charge, pH,...)
2)longer gel or shorter migration of different concentration of gellling.

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Post by JackBean » Mon Mar 21, 2011 11:05 pm

you could ask on your previous post...

Cis or trans? That's what matters.

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