HELP!!bacterial trasformation

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
agluke
Garter
Garter
Posts: 1
Joined: Fri Mar 18, 2011 10:50 am

HELP!!bacterial trasformation

Post by agluke » Fri Mar 18, 2011 10:53 am

Hi!
I have trasformed DH5alpha competent bacteria with a plasmid, and after the heat shock I resuspended them in YPD instead LB!!! Will it work anyway??

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Fri Mar 18, 2011 11:39 am

Gosh Darn it!
You wanted tuse LB? All protocols I have ever done use SOC which is richer. Your transformation is going to the weeds my friend. Or not.
You might have decreased a little bit the efficiency, but YPD is quite the rich medium anyway, so I guess they should recover nicely. Do not worry.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Mon Mar 28, 2011 10:10 am

:lol: saved an experiment by mistake :lol:
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Post Reply

Who is online

Users browsing this forum: No registered users and 3 guests