Reporter Genes

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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id10t
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Reporter Genes

Post by id10t » Sat Mar 12, 2011 5:45 am

How do you know what reporter gene to use when setting up a deletion analysis, or any other experiment?

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JackBean
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Post by JackBean » Sat Mar 12, 2011 8:56 am

usually you use the one, with which you have already experiences. And of course also something what you have equipment for. E.g. if you do not have fluorescent microscope, you probably won't want to use GFP ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

id10t
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Re: Reporter Genes

Post by id10t » Sat Mar 12, 2011 8:52 pm

o so a reporter gene is just a tag...it works with any gene?

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JackBean
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Post by JackBean » Sat Mar 12, 2011 9:44 pm

tag and reporter gene are something different
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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